ⅠNucleic asidhiiNhanganyaya
Nucleic acid yakakamurwa kuita deoxyribonucleic acid (DNA) uye ribonucleic acid (RNA), pakati peiyo RNA yakakamurwa kuita ribosomal RNA (rRNA), mutumwa RNA (mRNA) uye kuendesa RNA (tRNA) zvinoenderana nemabasa ayo akasiyana.DNA inonyanya kunyura munucleus, mitochondria uye chloroform, nepo RNA inonyanya kugoverwa mucytoplasm.Sehwaro hwezvinhu zvejini kutaura, nucleic acid kudhirowa kunoita basa rakakosha zvakanyanya mukutsvagisa mamorekuru biology uye kiriniki yemamomeru.Iko kusungirirwa uye kuchena kweiyo nucleic acid kudhirowa kuchakanganisa zvakananga iyo inotevera PCR, kutevedzana, kuvaka vector, enzyme digestion uye zvimwe zviedzo.
Ⅱ Nucleic acid kubviswa uye nzira yekuchenesa
① Phenol/chloroform yekubvisa nzira
Phenol/chloroform extraction inzira yechinyakare yekubvisa DNA, iyo inonyanya kushandisa maviri akasiyana organic solvents kurapa samples, kunyungudutsa DNA based nucleic acid muchikamu chemvura, lipids mu organic phase, uye mapuroteni pakati pezvikamu zviviri izvi.Iyi nzira ine zvakanakira mutengo wakaderera, kuchena kwakanyanya uye kuita kwakanaka.Izvo zvisingabatsiri ndezvekushanda kwakaoma uye nguva yakareba.
② Trizol nzira
Trizol nzira inzira yekare yeRNA kutorwa.Nzira yeTrizol yakakamurwa kuva chikamu chemvura uye chikamu chehupenyu mushure mekuisa centrifugation nekloroform, umo RNA inonyungudutswa muchikamu chemvura, chikamu chemvura chinotamirwa kune itsva EP tube, kunaya kunowanikwa mushure mekuwedzera isopropanol, uye ipapo ethanol kucheneswa.Iyi nzira inokodzera RNA kutorwa kubva kumhuka tishu, maseru uye mabhakitiriya.
③ Centrifugal column yekuchenesa nzira
Centrifuge mbiru yekuchenesa nzira inogona adsorb DNA zvakananga kuburikidza yakakosha silicon matrix adsorption zvinhu, nepo RNA neprotein inogona kupfuura zvakanaka, uyezve kushandisa yakakwira munyu yakaderera PH kusanganisa nucleic acid, yakaderera munyu yakakwira PH kukosha elution kuparadzanisa uye kuchenesa nucleic acid.Zvakanakira kucheneswa kwepamusoro, kugadzikana kwakanyanya, hapana chikonzero cheorganic solvent, uye mutengo wakaderera.Izvo zvakashata ndezvekuti inoda kuve centrifuged nhanho nhanho, mamwe matanho ekushanda.
④ Magnetic bead nzira
Iyo magineti beads nzira ndeyekutsemura sero tissue sample kuburikidza ne lysate, kuburitsa iyo nucleic acid mumuenzaniso, uyezve nucleic acid mamorekuru anonatswa adsorbed pamusoro peiyo magineti bead, nepo tsvina yakaita semapuroteni neshuga inosara mukati. mvura.Kuburikidza nenhanho dzesero kupatsanurwa kwematishu, magineti bead kusunga ne nucleic acid, nucleic acid washing, nucleic acid elution, nezvimwewo, yakachena nucleic acid inozopedzisira yawanikwa.Zvakanakira zviri nyore kushanda uye nguva pfupi yekushandisa, pasina kudiwa kwedanho centrifugation.Iyo ine yakaderera tekinoroji zvinodiwa uye inogona kuona otomatiki uye misa mashandiro.Iyo chaiyo musanganiswa weiyo magineti bead uye nucleic acid inoita iyo yakabviswa nucleic asidhi ine yakanyanya kugadzikana uye kuchena.Izvo zvakashata ndezvekuti mutengo wemusika wezvino unodhura.
⑤ Dzimwe nzira
Kuwedzera kune nzira ina dziri pamusoro apa, kune kufashaira kutsemuka, yakadzika munyu nzira, anionic detergent nzira, ultrasonic nzira uye enzymatic nzira, nezvimwe.
Ⅲ Mhando ye nucleic acid kubviswa
Foregene ine inotungamira pasi rose Direct PCR chikuva, kaviri-column RNA Isolation platform (DNA-chete + RNA chete).Zvigadzirwa zvikuru zvinosanganisira DNA/RNA isolation kits, PCR uye Direct PCR reagents molecular lab reagents akatevedzana.
① Yese RNA kutorwa
Yese RNA yekubvisa samples inosanganisira ropa, masero, mhuka dzemhuka, zvidyarwa, mavhairasi, etc. Kuchena kwepamusoro uye huwandu hwehuwandu hweRNA hunogona kuwanikwa kuburikidza neRNA yakazara kudhirowa, iyo inogona kushandiswa muRT-PCR, chip analysis, in vitro dudziro, molecular cloning, Dot Blot uye zvimwe zviedzo.
Foregene relatedRNA Isolation Kits
Mhuka Yese RNA Isolation Kit--Nekukurumidza uye zvinobudirira bvisa yakakwira-kuchena uye yemhando yepamusoro RNA kubva kune akasiyana mhuka tishu.
Sero Yese RNA Isolation Kit--Yakanyanya kucheneswa uye yemhando yepamusoro RNA inogona kuwanikwa kubva kune akasiyana siyana maseru mumaminetsi gumi nerimwe.
Plant Yese RNA Isolation Kit--Kurumidza bvisa yemhando yepamusoro RNA kubva kumasampu echirimwa ane yakaderera polysaccharide uye polyphenol zvemukati.
Viral RNA Isolation Kit--Kurumidza kuparadzanisa uye kuchenesa hutachiona RNA kubva kumasampuli akadai seplasma, serum, sero-isina zvinwiwa zvemuviri uye sero tsika supernatants.
② Genomic DNA kutorwa
Genomic DNA extraction samples inosanganisira ivhu, tsvina, ropa, masero, mhuka dzemhuka, zvirimwa, mavhairasi, etc. Genomic DNA extraction inogona kushandiswa enzyme digestion, DNA library library, PCR, antibody kugadzirira, Western blot hybridization analysis, gene chip, yakakwirira. -kutevedzana kwemaitiro uye zvimwe zviedzo.
Foregene relatedDNA Isolation Kits
Animal Tissue DNA Isolation Kit--Kukurumidza kutorwa uye kucheneswa kwegenomic DNA kubva kune akawanda masosi, senge mhuka tishu, maseru, nezvimwe.
Ropa DNA Midi Kit (1-5ml)--Kurumidza kuchenesa yepamusoro-mhando genomic DNA kubva kune anticoagulated ropa (1-5ml).
Buccal Swab/FTA Kadhi DNA Isolation Kit--Kurumidza kuchenesa yemhando yepamusoro genomic DNA kubva kune buccal swab/FTA Kadhi samples.
Plant DNA Isolation Kit--Kurumidza kuchenesa uye uwane yakakwirira-mhando genomic DNA kubva kumiti samples (kusanganisira polysaccharides uye polyphenol chirimwa samples)
③ Kubvisa plasma
Plasmid imhando yedenderedzwa diki molecule DNA mumaseru, inova yakajairwa kutakura DNA recombination.Nzira yekubvisa plasmid ndeyekubvisa RNA, kupatsanura plasmid kubva kubhakitiriya genomic DNA, uye kubvisa mapuroteni nezvimwe zvinosvibisa kuti uwane plasmid yakachena.
General Plasmid Mini Kit--Kurumidza kuchenesa yemhando yepamusoro plasmid DNA kubva kune yakashandurwa mabhakitiriya kune yenguva dzose mamorekuru biology kuedza sekushandura uye kugaya enzyme.
④ Mamwe marudzi ekubvisa, miRNA kuburitsa, nezvimwe.
Mhuka miRNA Isolation Kit--Nekukurumidza uye nekunyatso bvisa zvimedu zvidiki zveRNA zve20-200nt miRNA, siRNA, snRNA kubva kune akasiyana matishu emhuka nemaseru.
Ⅳ Zvinodiwa zve nucleic acid kubviswa uye mhedzisiro yekucheneswas
① Kuve nechokwadi chekuvimbika kweiyo yekutanga chimiro che nucleic acid.
② Deredza kupindira kwemapuroteni, shuga, lipids uye mamwe macromolecules
③ Panofanira kunge pasina organic solvent kana yakanyanya kuwanda kwesimbi iyoni inogona kuvharira iyo enzyme mune nucleic acid samples.
④ RNA uye kumwe kusvibiswa kwenyucleic acid kunofanirwa kubviswa kana uchibvisa DNA, uye zvinopesana.
Nguva yekutumira: Nov-24-2022
